Download e-book for iPad: Antigen Processing: Methods and Protocols by Stefan Tenzer, Tobias Hain, Hendrik Berger, Hansjörg Schild

By Stefan Tenzer, Tobias Hain, Hendrik Berger, Hansjörg Schild (auth.), Peter van Endert (eds.)

ISBN-10: 1627032177

ISBN-13: 9781627032179

Antigen processing is a organic technique that prepares antigens for the presentation to important cells within the immune process known as T lymphocytes. In Antigen Processing: equipment and Protocols, specialist researchers within the box supply a accomplished set of protocols for learning presentation of antigens produced within the typical processing pathways for MHC classification I and sophistication II molecules. The chapters stick with chronology of intracellular processing occasions, finishing with acceptance of peptide-MHC complexes on the mobilephone floor via T lymphocytes. Written within the hugely profitable Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, with ease reproducible laboratory protocols, and key pointers on troubleshooting and heading off identified pitfalls.

Authoritative and functional, Antigen Processing: tools and Protocols is designed for newbies and specialists attracted to learning antigen processing.

Show description

Read or Download Antigen Processing: Methods and Protocols PDF

Similar immunology books

New PDF release: Monoclonal Antibody Technology: The Production and

This quantity comprises distinctive, complete recommendation on rat, mouse and human hybridoma construction. It starts off with a normal advent, then describes the sensible purposes of the expertise with images and protocols for every thing from animal dissection to epitope research of antigens.

Immunology: Mucosal and Body Surface Defences - download pdf or read online

Nearly all of medically very important pathogens infect their host throughout a physique floor resembling the outside, or throughout a mucosal tissue corresponding to the breathing tract or intestines, as those websites are those uncovered to the exterior atmosphere. via targeting immunity at mucosal and physique surfaces this publication provides a clean, new method of the educating of immunology.

Lentiviral Vectors and Gene Therapy by David Escors, Karine Breckpot, Frederick Arce, Grazyna PDF

Gene remedy was once conceived throughout the early and mid a part of the twentieth century. initially, it was once thought of a progressive biomedical strategy, which may in all probability therapy any disorder for which the molecular bases have been understood. due to the fact that then, gene treatment has passed through many levels and has advanced from an almost unrealistic point of view to a true lifestyles software.

Immunology and Drug Therapy of Allergic Skin Diseases - download pdf or read online

Allergic pores and skin ailments belong to the commonest dermatoses. This ebook bargains with primary (in specific immunological features) in addition to medical indicators and healing recommendations of the allergic dermis illnesses. Cells all in favour of the pathomechanisms of allergic dermis illness are defined in person chapters.

Additional info for Antigen Processing: Methods and Protocols

Sample text

Antigen Processing: Methods and Protocols, Methods in Molecular Biology, vol. 1007/978-1-62703-218-6_2, © Springer Science+Business Media, LLC 2013 15 16 K. Textoris-Taube et al. the presence of immunoproteasomes and PA28 (5–10) influence epitope liberation either positively or negatively. To study proteasomal antigen processing simple in vitro systems have been established that allow mimicking the in vivo situation with surprisingly high fidelity. These assays generally involve the processing of a synthetic polypeptide of 20–30 amino acid residues in length that harbor a known or a potential MHC class I epitope by purified 20S proteasomes, HPLC-separation of the generated peptides followed by MS analysis of the generated peptide products (11) (see Note 1).

You need an appropriate laser in your flow cytometer to excite your fluorescent dye and also detectors measuring the correct emission wavelength. 38 M. Basler and M. Groettrup 14. Do not count residual un-lysed erythrocytes. 15. It is useful to pulse female splenocytes with different concentrations of synthetic peptide (10−5 to 10−10 M) and use the pulsed cells as stimulators for hybridomas. Comparison of the stimulation of the titrated peptide probes with your samples will provide information regarding the magnitude of observed differences.

Nat Immunol 11:449–454 3. Stoltze L, Schirle M, Schwarz G et al (2000) Two new proteases in the MHC class I processing pathway. Nat Immunol 1:413–418 4. Saric T, Beninga J, Graef CI et al (2001) Major histocompatibility complex class I-presented antigenic peptides are degraded in cytosolic extracts primarily by thimet oligopeptidase. J Biol Chem 276:36474–36481 5. York IA, Mo AXY, Lemerise K et al (2003) The cytosolic endopeptidase, thimet oligopeptidase, destroys antigenic peptides and limits the extent of MHC class I antigen presentation.

Download PDF sample

Antigen Processing: Methods and Protocols by Stefan Tenzer, Tobias Hain, Hendrik Berger, Hansjörg Schild (auth.), Peter van Endert (eds.)


by Steven
4.0

Rated 4.88 of 5 – based on 28 votes